How TaqMan Works -- Ask TaqMan® Ep. 13

Submit your real-time PCR questions https://www.thermofisher.com/us/en/home/life-… Just how does TaqMan work? Sr. Field Applications Specialist Doug Rains explores the specific mechanism by which TaqMan® achieves its unparalleled specificity and sensitivity. Around the world, researchers rely on TaqMan® for gene expression, SNP gentoyping , protein expression, pathogen detection and quantification, and more. For many years, TaqMan has been the gold-standard chemistry for real-time PCR. It's famed for its unparalleled specificity, sensitivity, and ease of use. So it's not surprising that users want to know what Shrikant at ICL College in India asked recently: namely, "How does TaqMan work?" I'm glad you asked. Just like any PCR, TaqMan-based reactions require a double-stranded template, as well as two fairly standard, target-specific primers. But unlike those used in regular PCR, Taq